About

Scientific focus was founded in  Copenhagen, Denmark, in 2015. We  relaized that the incredible  development in the field of microscopy and the many new opportunities on offer made it  challenging to actually find the best imaging  strategy for a specific project. Our principle is  to use our background and extensive  experience in microscopy, image processing  and molecular biology to find the optimal  solution for our customer’s project.

Johannes Liesche, PhD

Johannes is the Founder and Director of  Scientific focus. He obtained his PhD in Cell  Biology from the University of Copenhagen  and has also worked at Cornell University,  Edinburgh University and Humboldt  University Berlin. Selected publications: Cell wall staining with Trypan Blue enables  quantitative analysis of morphological  changes in yeast cells. Liesche J, Marek M,  Günther- Pomorski T. (2015)  Frontiers in  Microbiology. This paper describes a novel staining method  that enables the quantification of  morphological parameters of living fungal  cells, mostly via tomographic imaging. Analysis of intended learning outcomes and  evaluation of teaching activities in practical  courses on microscopy. Liesche J, In  Christiansen F (ed.) Improving University  Science Teaching and Learning. Vol 8, No 1-2.  University of Copenhagen Press, Copenhagen  (2015).  A book chapter on how to teach microscopy  most efficiently. Super-resolution imaging with Pontamine  Fast Scarlet 4BS enables direct visualization of cellulose orientation and cell connection  architecture in onion epidermis cells. Liesche  J, Ziomkiewicz I, Schulz A. (2013)  BMC Plant  Biology The first paper to investigate the  structure of cell walls at single-molecule  resolution. Unravelling the structure of viral replication  complexes at super-resolution. Linnik O,  Liesche J, Tilsner J, Oparka K. (2013)  Frontiers  in Plant Science. One of the first papers to show the 3D  structure that viral proteins form inside cells  at a resolution below 100 nm. Quantification of plant cell coupling with  three- dimensional photoactivation  microscopy. Liesche J, Schulz A. (2012)  Journal of Microscopy. The first method to measure diffusion  coefficients for small molecules transported  between cells in living plant tissue is  presented in this paper. 

Experience

With 10 years of experience in  advanced microscopy, we know how  to approach a project. We regularly  use a very wide range of instruments  and microscopy methods. 

Excellence

Our team has successfully concluded  numerous research projects, as  evidenced by a high number of  publications in international journals. As research leaders, we have ample  experience with science management.  and also worked as Journal editors for important international journals.

Dedication

As scientists with an academic  background we are familiar with the  pressure to realize a project as fast as  possible. We are also used to the  scientific working culture and are fully committed to put our full dedication  behind your project.